Stat3 phospho-y705 kit

Author: tdcx

August undefined, 2024

WebDec 21, 2012 · Five hundred nanograms of DNA were bisulfite-converted with the Epitect Bisulfite Kit (Qiagen) according to the manufacturer's instructions. ... phospho-STAT3 Y705 (1:50; Cell Signaling Technology), phospho-STAT3 S727 (1:50; Cell Signaling Technology), human nucleolin (1:1000; Millipore), and human nuclear antigen (1:20; Chemicon). … WebClone 4/P-STAT3 (RUO) View all Formats Flow cytometric analysis of Stat3 (pY705). Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated (unshaded) or stimulated (shaded) with recombinant human IL-6 (MN 550071) at 100 ng/mL for 15 min at 37 °C.

Phosphorylated STAT3 (Y705) LANCE Ultra Cellular …

WebAn anti-pan STAT3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT3 present in a sample is bound to the wells by the immobilized … WebProduct Description. CST's PathScan ® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects … ra 2727

Ginsenoside Rb1 ameliorates the abnormal hepatic

WebSTAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon … WebSummary for Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA. This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human and mouse STAT3 (Y705) in cell lysates. An immobilized capture antibody specific for STAT3 binds both phosphorylated and unphosphorylated … WebJun 25, 2016 · Antibodies phospho-STAT3(Y705), STAT3, cleaved caspase-3, cleaved PARP, phospho-p65, phospho-STAT1 (Y701), STAT1, phospho-JAK2 (Y1007/1008), JAK2, phospho- JAK1 (Y1022/1023), JAK1, phosphor-JNK (Y183/Y185), phosphor-ERK (T202/Y204), GAPDH HRPconju- gated secondary antibodies were from Cell Signaling Technology (Beverly, … don\\u0027t give up

THUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling …

Cisbio 62AT3PEG STAT3 phospho-Y705 kit, 500 Tests

WebSTAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. WebJun 25, 2016 · Antibodies phospho-STAT3(Y705), STAT3, cleaved caspase-3, cleaved PARP, phospho-p65, phospho-STAT1 (Y701), STAT1, phospho-JAK2 (Y1007/1008), JAK2, … don\u0027t give up 2021WebPathScan ® Phospho-Stat3 (Tyr705) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Stat3 (Tyr705) in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. Background ra 272

"WebWe found that NSC74859 inhibited p-STAT3(Y705) in SHH MB cells in a dose dependent manner and reduced the levels of EMT markers N-cadherin and slug compared to the DMSO control (Fig. 6 a, b & Additional file 1: Fig. S11a, b). We also analyzed STAT3 and STAT3 Y705 phosphorylation in IGFBP2 knockdown cells after stimulation with exogenous IGFBP2. " - Stat3 phospho-y705 kit

Stat3 phospho-y705 kit

Anti-Stat3 (phospho-Y705) Antibody (A24894) Antibodies.com

WebApr 14, 2024 · Phospho-STAT3 (Y705) Antibody - Prepare a 100X working concentration of the antibody by reconstituting with 110 L of 1X PBS. After reconstitution, store at 2-8° C … WebApr 12, 2024 · Y705-STAT3 and p65 acted additively in reducing CSC enrichment, and Y705A-STAT3 variant + sip65 has enriched chemo-resistant CSCs. ... Millipore, Temecula, …

Did you know?

WebSTAT3 (Phospho) [pY705] Human InstantOne™ ELISA Kit. Representative data for STAT3 (Phospho) [pY705] Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 … WebSTAT3 phospho Y705 Antibody Rabbit Polyclonal Item No. 600-401-C64 Size: 100 µg Applications: ELISA, IHC, WB Reactivity: Human Host Species: Rabbit Have a Question? Ask a Specialist CONTACT US OR +1 484.791.3823 $369.00 /Per Item 25 µL $125.00 100 µg $369.00 Add To Cart Add to List Bulk Orders Availability: Ships next business day …

WebTHUNDER™ Phospho-STAT3 (Y705) TR-FRET Cell Signaling Assay Kit Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT3 (Y705) in cell lysates. … WebThe antibody pair provided in this kit recognizes human, mouse and rat Phospho-Stat3 (pTyr705). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen …

WebMar 28, 2024 · Product Description. Htrf cellular assay kit for measuring phosphorylated STAT3 directly in cells based on our homogeneous and robust htrf assays, the phospho-stat3 Kit is designed for detecting and studying activated STAT3 when phosphorylated at Tyr705 directly in whole cells. Using a streamlined protocol, amenable to Low-volume … WebMar 28, 2024 · Product Description. Htrf cellular assay kit for measuring phosphorylated STAT3 directly in cells based on our homogeneous and robust htrf assays, the phospho …

WebCell-Based phospho-STAT ELISA Samper Kit for detecting phospho-STAT1 (pTyr 701), phospho-STAT3 (pTyr 705), and phospho-STAT5 (pTyr 694) in cultured cell lines 检测培养细胞系中磷酸化-STAT1（pTyr(pTyr 701),磷酸化-STAT3 (pTyr 705), 和磷酸化-STAT5 (pTyr 694)的基于细胞的ELISA试剂盒

WebApr 14, 2024 · Phospho-STAT3 (Y705) Antibody - Prepare a 100X working concentration of the antibody by reconstituting with 110 L of 1X PBS. After reconstitution, store at 2-8° C for up to 30 days or ... phosphorylation by Western blotting using the antibodies supplied in the kit is also shown (inset). 8. Figure 2: Dose response of pervanadate-induced STAT3 ... ra 2729WebJan 8, 2024 · STAT3 activation involves the phosphorylation of a critical tyrosine residue, Tyr705. Phosphorylation of Tyr705 results in homo- or hetero-dimerization of STAT3, enabling nuclear localization... don\u0027t give a tinker\u0027s damWebHuman Phospho-STAT3 (Y705) Antibody Summary Species Reactivity Human Specificity Detects human STAT3 when phosphorylated at Y705. Source Polyclonal Rabbit IgG Purification Antigen Affinity-purified Immunogen Phosphopeptide containing human STAT3 Y705 site Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. don\u0027t give up biblehttp://www.biotnt.com/product/product_799431.html don\u0027t give up 2 gameWebQuality Control: the Phospho-STAT3 (Y705) + Total STAT3 assay kit is routinely tested against IFNα-treated HeLa lysates. HeLa cells were cultured in a T175 flask to 90% confluence and stimulated with 3 nM of IFNα for 30 min at RT. Following cell lysis using 4 mL of 1X Lysis Buffer 1, ra 27/3WebMar 23, 2024 · Phospho-STAT3 (Tyr705) and Total STAT3 ELISA Kit Catalog #: PEL-Stat3-Y705-T User Manual Last revised March 23, 2024 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA 30092 Tel: 1-888-494-8555 (Toll Free) or 770-729-2992, Fax:770-206-2393 Web: www.RayBiotech.com, … ra272 製作WebPhospho-Stat3-Y705 Antibody kit (RK04099) Datasheet Tested applications: Reactivity: Human, Mouse Datasheet Reviews Protocols Inquire About This Product Overview Product Component Background The protein encoded by this gene is … don\u0027t give up brandon lake