Phic31 integrase system

Author: crfw

August undefined, 2024

WebϕC31 integrase can integrate a donor plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable. The ϕC31 … WebUnlike the better-known recombinases such as Cre and Flp, PhiC31 integrase catalyzes recombination between two nonidentical sites and lacking a corresponding excision …

Expression of active Streptomyces phage phiC31 integrase in …

Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 Web10. apr 2024 · The phiC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition ... deleting recovery partition

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Web29. júl 2012 · The phiC31 integrase system is thought to be more efficient than the FLP recombinase for the construction of transgenic insects because it has been successfully applied to diverse organisms, such as humans, flies, and mosquitoes (Groth et al. 2000, 2004; Bateman et al. 2006; Nimmo et al. 2006; Bischof et al. 2007 ). WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … Web17. mar 2006 · The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also … ferme yves bonin

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Improved site-specific recombinase-based method to produce

WebThe objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embryos by somatic cell nuclear transfer and intracytoplasmic sperm injection. Materials and Methods: In this experimental study, the application of phiC31 integrase system was evaluated for Web25. júl 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. fermez la bouche translationWeb28. feb 2014 · PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in … deleting recovery partition on flash drive

"WebThe PhiC31 Integrase System. Frequently used for gene therapy development and related applications; Non-viral transgene delivery; Single-step process; Single-copy integration; … " - Phic31 integrase system

Phic31 integrase system

A rapid and bidirectional reporter of neural activity reveals neural ...

WebBackground: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous... Web4. mar 2016 · Use of phiC31 Integrase for Constructing Transgenic Organisms One application of phage integrases that has been popular is the use of phiC31 integrase to place transgenes into the genome to construct transgenic organisms. Both pseudo att sites and authentic att sites have been used in this regard.

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Web14. sep 2012 · The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination. We produced transgenic barley plants expressing an active phiC31 integrase and crossed them with transgenic barley plants carrying a target locus for recombination. The target … Web29. júl 2012 · The phiC31 integrase system. is thought to be more efﬁcient than the FLP recombinase for. the construction of transgenic insects because it has been. successfully applied to diverse organisms ...

WebTo overcome these problems, it is necessary to develop a site-specific integration system. It is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the silkworm, we first examined the efficiency of recombination between the ... WebWe previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors.

WebTwo orthogonal systems (MS2 and PP7) have been adopted for Drosophila [9-11]. Nascent mRNAs transcribed from the tagged loci carry stem-loops at their 5′ ends, preferably intronic regions. ... We used BDSC #34770 as the maternal phiC31 integrase source. These female flies provide integrase activity in their germlines, which induces attB/attP ... WebUstawienia Tekstu. 1 Odstęp między wierszami. 1 Odstęp między paragrafami

WebThe phiC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB …

WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the … ferme yvrenchWebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ... deleting recovery partition diskpartWeb17. sep 2008 · The phiC31 integrase system is expected to be functional in other monocotyledonous species and in commercial wheat varieties. We succeeded in transforming phiC31 integrase vectors into rice ( Oryza sativa ), pearl millet ( Pennisetum glaucum ) and varieties of commercial winter wheat provided by the Nordsaat Saatzucht … ferme yves landryWeb• Developed phiC31 integrase system in Hydra to optimize transgenesis via site-specific recombination. • Designed DNA constructs for microinjections. • Mentored undergraduate students and ... deleting recycle bin iconWeb1. dec 2006 · The phiC31 integrase can integrate introduced plasmid DNA into preferred locations in unmodified mammalian genomes, resulting in robust, long-term expression of … ferm factoryWeb1. feb 2005 · PhiC31 integrase system is considered as a specific tool for gene therapy (3, 4) and transgenic research (2,5). The efficiency of phiC31-integrase has been indicated to be comparable with that of ... fermfast alpha amylasehttp://www.systembio.com/wp/wp-content/uploads/phiC31_productsheet-1.pdf deleting remote branch in git